This workshop focuses on the super-resolution technique of STochastic Optical Reconstruction Microscopy (STORM), particularly Direct STORM (dSTORM) and the analogous techniques of Ground State Depletion Imaging Microscopy (GSDIM) and Nikon STORM (N-STORM).
Monash Micro Imaging workshops emphasise hands-on learning and provide practical training. Lectures are designed to illustrate the practical aspects of techniques and everyone attending the practical sessions is expected to gain hands-on experience in preparing samples, operating microscopes and optimising imaging parameters to gain good data, and performing localisation analysis.
Concepts discussed in the Analysis lectures and practical sessions are applicable to other single molecule localisation techniques, such as PALM.
Date: 17-19 August 2015
Target audience: research staff and students working with Super Resolution by Single Molecule Localisation, specifically STORM and STORM variants.
Contact time
Lectures: 6hrs
Instrumentation and Analysis: 9hrs
Instructors
The instructors are experts who work regularly in the lab with these techniques:
Dr Kirstin Elgass (MMI), Dr Betty Kouskousis (Burnet Institute/MMI), Dr Catherine Palmer (Burnet Institute/MMI), Dr Keith Schulze (MMI) and Dr Carl Ebeling (Bruker).
Instrumentation
Monash-built dSTORM system (Clayton), Bruker Vutara SR350 instrument (Clayton),
Software: RapidSTORM, ThunderSTORM.
Workshop Registration
By application/expression of interest:
https://facultyapplications.wufoo.eu/forms/superresolution-by-storm-2015/
Recommended reading for beginners:
1. Q&A: Single-molecule localization microscopy for biological imaging, McEvoy et al.
http://www.biomedcentral.com/1741-7007/8/106
2. A starter kit for point-localization super-resolution imaging. Manley et al.
DOI 10.1016/j.cbpa.2011.10.009
Workshop Conveners and for further information contact:
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