The system employs an RNA-guided nuclease to facilitate sequence-specific cleavage of target loci, allowing gene disruption or editing by homologous recombination.
Genome modifications can be made by direct injection into fertilised mouse oocytes, resulting in the rapid and efficient generation of founder mice.
Following a thorough evaluation of the technology through a number of pilot projects, the Monash University node of the Australian Phenomics Network (APN) can now offer CRISPR/Cas9 genome editing as an efficient way to generate custom mouse models for clients. Our staff will design and produce guide RNAs, inject the RNAs into oocytes, perform the initial mutation screening of the founder mice and coordinate the shipment of targeted animals to your animal facility.
For further information contact our Facility Manager, Dr Leanne Hawkey (firstname.lastname@example.org) to learn more about this exciting new service!